Part:BBa_K4759051:Design

petH-linker-petF-linker-Olep

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 1022
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1551
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1606
  Illegal AgeI site found at 1704
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The petH, petF, and Olep were all ligated by linker and expressed by fusion

Source

ferredoxin reductase PetH (SEQ ID NO.7) and ferredoxin PetF (SEQ ID NO.8) from the algae (Synechocystis PCC 6803)